Table 1 Human primers for qRT-PCR
Gene | Primer | Sequence |
|---|---|---|
H3F3A | Human cDNA forward | ACAAAAGCCGCTCGCAAGAGTG |
Human cDNA reverse | TTTCTCGCACCAGACGCTGGAA | |
INS | Human cDNA forward | GCAGCCTTTGTGAACCAACAC |
Human cDNA reverse | CCCCGCACACTAGGTAGAGA | |
NGN3 | Human cDNA forward | CTAAGAGCGAGTTGGCACTGA |
Human cDNA reverse | GAGGTTGTGCATTCGATTGCG | |
PDX1 | Human cDNA forward | GAAGTCTACCAAAGCTCACGCG |
Human cDNA reverse | GGAACTCCTTCTCCAGCTCTAG | |
SOX9 | Human cDNA forward | AGGAAGCTCGCGGACCAGTAC |
Human cDNA reverse | GGTGGTCCTTCTTGTGCTGCAC | |
CK19 | Human cDNA forward | AGCTAGAGGTGAAGATCCGCGA |
Human cDNA reverse | GCAGGACAA TCCTGGAGTTCTC | |
AMY2A | Human cDNA forward | GATAATGGGAGCAACCAAGTGGC |
Human cDNA reverse | CAGTATGTGCCAGCAGGAAGAC | |
MAFA | Human cDNA forward | GCTTCAGCAAGGAGGAGGTCAT |
Human cDNA reverse | TCTGGAGTTGGCACTTCTCGCT | |
INS hnRNA | Human cDNA forward | GAGATGGGGAAGATGCTGGG |
Human cDNA reverse | GGAGGACACAGTCAGGGAGA | |
EZH2 | Human cDNA forward | TCCTTTTCATGCAACACCCAACACT |
Human cDNA reverse | TCCAAATGCTGGTAACACTGTGGTC | |
EP300 | Human cDNA forward | GCAGTGTGCCAAACCAGATG |
Human cDNA reverse | GGGTTTGCCGGGGTACAATA | |
KAT2 | Human cDNA forward | ATTCTGCAGGGGCCGAGCCT |
Human cDNA reverse | ATCACACGGAGCCGCTTGGC | |
BRG1 | Human cDNA forward | GCTCATGGCTGAAGATGAGG/ |
Human cDNA reverse | CAGGCGCTTGTCCTTCTTC |