Fig. 2

Key markers of endocrine development, epithelial-to-mesenchymal transition, pancreatic ducts, and islets are altered in human pancreatic ductal cells following stimulation with EZH2 inhibitors. Variation in mRNA transcripts of A Neurogenin3 [NGN3], B Pancreatic duodenal homeobox factor 1 [PDX1], C SRY-box transcription factor 9 [SOX9], D Alpha amylase [AMY2A], E Cytokeratin 19 [CK19], F Insulin heterogeneous nuclear RNA [INS hnRNA], G Insulin [INS] and H V-maf musculoaponeurotic fibrosarcoma oncogene homolog A [MAFA], following 2- and 7-day stimulation with GSK-126 at 10 µM, EPZ6438 at 1 µM, and triptolide at 20 nM. Data are displayed as mean of fold change ± S.E.M. of 3 replicates, calculated by normalizing drug values to DMSO (vehicle-treated) controls. Statistically significant change in expression was determined using Student’s t test to compare control values to each drug, *P < 0.05, **P < 0.01, ***P < 0.001, ****P < 0.0001