Assessment of immune cell profiles among post-menopausal women in the Women’s Health Initiative using DNA methylation-based methods

Table 3 Comparing immune cell counts to those in the literature (selected version)

Study	WHI (this study)	Provinciali et al. [17]^a	Thyagarajan et al. [1]^b	Valiathan et al. [11]^c	Melzer et al. [3]^d
Geographic area	United States	Italy	United States	United States	Germany
Sex	Women	Women	Women	Women	Women
Age range	50–94	46–90+	56–86+	70–92	60–79
Sample size	1294	191	4874	33	195

Method		DNAm deconvolution			Flow cytometry	Flow cytometry	Flow cytometry		Flow cytometry
Type		Percentile (2.5–97.5)	Mean	Median	Weighted mean	Weighted mean	Mean	Median	Median	Percentile (2.5–97.5)
Neutrophils	Cells/µl	(1334, 7202)	3642	3470					3122	(1801, 5579)
Eosinophils	Cells/µl	(19, 444)	140	112					101	(30, 383)
Monocytes	Cells/µl	(262, 961)	546	524					230	(108, 422)
Lymphocytes	Cells/µl	(842, 3621)	1921	1823					1624	(677, 2916)
T cells	Cells/µl	(525, 2646)	1358	1280		1434	1424	1351	1098	(369, 2086)
CD4+ total	Cells/µl	(296, 1799)	860	795	710	999	892	867	818	(263, 1707)
CD4+ memory	Cells/µl	(171, 1328)	610	564	324	384
CD4+ naïve	Cells/µl	(35, 686)	201	132	176	486
T regulatory	Cells/µl	(27, 88)	50	47					60	(22, 130)
CD8+ total	Cells/µl	(85, 1473)	498	397	410	337	525	453	143	(32, 437)
CD8+ memory	Cells/µl	(37, 1386)	418	306	178	200
CD8+ naïve	Cells/µl	(28, 247)	80	58	59	73
B-total	Cells/µl	(50, 640)	227	189			229	164	216	(62, 513)
Natural Killer	Cells/µl	(95, 762)	335	308			339	250	276	(85, 624)
CD4/CD8		(0.35, 11.1)	3.01	2.07			2.4	1.7

^aWeighted means were calculated from data in Table 1 of Provinciali et al.
^bWeighted means were copied from Additional file 1: Table S3 of Thyagarajan et al. and converted to cells/µl; CD4mem and CD8mem were added from their respective TCM + Effector + TEM compartments
^cMean and median values were copied from Additional file 1: Table S2 of Valiathan et al.
^dMedian and percentile values were copied from Additional file 1: Table S4 of Melzer et al. and converted to cells/µl; CD4 were taken from T helper (Th) cells, CD8 were taken from cytoxic T (Tc) cell

ISSN: 1868-7083