Fig. 5

Sequencing analysis of KGN cells m6A. A, B The overexpression METTL3 plasmid was constructed. 24 h after it was transferred into KGN cells, the protein expression level of METTL3 was detected. The results showed that the expression of METTL3 in the overexpression plasmid group (OE) was significantly higher than that in the blank plasmid group (NC). Bars represent means ± SD, n = 3. *P < 0.05. C MRNA level of METTL3 increased significantly in the overexpression group. Bars represent means ± SD, n = 3. *P < 0.05. D Distribution of m6A fragments enriched by two groups of KGN cells in RNA, most of which are located in 3’UTR region. E Quantification analysis of m6A modification in the KGN cells of the METTL3-NC and METTL3-OE group. The m6A content in total RNA of KGN cells were detected by colorimetric assay. Bars represent means ± SD, n = 3. *P < 0.05 vs the control. F Metagene profiles of enrichment of m6A peaks across mRNA transcriptome of the METTL3-NC and METTL3-OE group. G Veen diagram shows that through enrichment analysis, eight potential targets are selected according to mRNA expression level, m6A modification level and motif region, and FOSL2 is determined as a target for subsequent research in combination with relevant literature. H The expression amount of FOSL2 mRNA motif1 in KGN cells of the two groups. qpcr results showed that when METTL3 was overexpressed in KGN, the expression of FOSL2 m6A modified motif1 increased. Bars represent means ± SD, n = 3. *P < 0.05