Fig. 1
From: Neuroglobin plays as tumor suppressor by disrupting the stability of GPR35 in colorectal cancer
NGB is downregulated due to promoter methylation in CRC A The mRNA expression and promoter methylation of NGB in COAD. COAD: colon adenocarcinoma, data from UALACN (http://ualcan.path.uab.edu/). B Protein level of NGB in paired human colorectal carcinoma tissues detected by IHC staining, N = 3. C Fold-change of NGB mRNA expression detected by qRT-PCR in CRC and CRC-A. CRC: CRC, CRC-A: CRC-adjacent. D RT-PCR and Western blot results showing mRNA expression of NGB in CRC cell lines. E Expression of NGB in normal colon, primary tumor, liver metastasis and lung metastasis of CRC, data from GSE412584. F Methylated analysis of NGB in CRC cell lines, p < 0.05. G The mRNA expression level of NGB detected by qRT-PCR after treatment with demethylating agent Aza. H Methylation status of NGB detected by qMSP after treatment with demethylating agent Aza. All experiments were performed in triplicate. *p < 0.05, **p < 0.01, ***p < 0.001