DNA methylation patterns at birth predict health outcomes in young adults born very low birthweight

Clinical Epigenetics

Table 4 CpGs differentially methylated in adult VLBW versus controls and associations with birthweight or gestation adjusting for study group, sex and ethnicity

CpG ID-nearest gene	Chromosome: location	p value	FDR-adj p value*	Fold change	R statistic	Direction in VLBW	Birthweight p value and direction (Full Cohort)		Gestation p value and direction (VLBW cases)
cg13518079 *EBF4*	20: 2,675,072	1.31E⁻⁰⁹	0.001	1.067	0.343	Hypermethylated	< 0.001	–	0.015	–
cg05857996 *EBF4*	20: 2,675,418	4.90E⁻⁰⁹	0.002	1.065	0.332	Hypermethylated	0.002	–	0.009	–
cg22891070 *HIF3A*	19: 46,801,642	1.25E⁻⁰⁸	0.003	0.942	− 0.323	Hypomethylated	0.013	+	0.012	–
cg16672562 *HIF3A*	19: 46,801,672	3.05E⁻⁰⁸	0.006	0.944	− 0.315	Hypomethylated	0.015	+	0.013	–
cg24263062 *EBF4*	20: 2,730,191	4.20E⁻⁰⁸	0.007	1.047	0.312	Hypermethylated	< 0.001^††	–	0.011	–
cg26344859 *KCNQ1*	11: 2,584,629	1.08E⁻⁰⁷	0.015	0.992	− 0.303	Hypomethylated	ns		ns
cg14486095 UNC119B**	12: 121,147,222	2.19E⁻⁰⁷	0.026	1.044	0.296	Hypermethylated	0.028^††	+	ns
cg04099095 *EVX1*	7: 27,278,570	3.19E⁻⁰⁷	0.029	1.013	0.292	Hypermethylated	0.049	–	ns
cg27146050 *HIF3A*	19: 46,801,557	3.35E⁻⁰⁷	0.029	0.978	− 0.291	Hypomethylated	0.005	+	ns
cg20219891 *GLI2*	2: 121,496,875	3.52E⁻⁰⁷	0.029	1.053	0.291	Hypermethylated	0.027	–	ns
cg05149986 *CFI*	4: 110,683,920	6.41E⁻⁰⁷	0.048	1.015	0.285	Hypermethylated	ns		0.007	–
cg05825244 *EBF4*	20: 2,730,488	7.23E⁻⁰⁷	0.049	1.068	0.283	Hypermethylated	< 0.001^††	–	0.005	–

*Benjamini–Hochberg FDR p value is corrected for 822,741 CpG sites. R statistic is correlation coefficient for continuous variables
**cg14486095 in UNC119B was found to be polymorphic and was excluded from further analyses
^††Following the p value indicates both the interaction term CpG x Study Group and CpG are significant p < 0.05

ISSN: 1868-7083