| | 5-mC/5-hmC Interaction (q < 0.2) | 5-mC Only (q < 0.05) | 5-hmC Only (q < 0.05) |
|---|
PFHxS | 105 | 80 | 101 |
PFOS | 1516 | 24 | 1388 |
PFOA | 637 | 1 | 0 |
PFNA | 2281 | 879 | 1684 |
PFDA | 8054 | 3527 | 7432 |
PFUnDA | 3103 | 361 | 2507 |
MeFOSAA | 272 | 164 | 264 |
- Counts of the number of CpG sites with statistically significant associations between each PFAS and methylation (n = 70 for all analyses). 5-mC/5-hmC-interaction models were used to select the sites to stratify by each type of methylation. The following models were fit, where bolded term indicates the estimate of interest generating the counts above:
- \(\begin{aligned} 5 - {\text{mC}}\;{\text{or}}\;5 - {\text{hmC}}\;{\text{proportion}}\;{\text{at}}\;{\text{528}},{\text{389}}\;{\text{Sites}} & = \beta _{0} + \beta _{1} {\text{PFAS}} + \beta _{2} {\text{Parity}} + \beta _{3} {\text{Smoking}} + \beta _{4} {\text{Race}} \\ & \quad + \beta _{5} {\text{sex}} + \beta _{6} {\text{CD}}4{\text{T}} + \beta _{7} {\text{CD}}8{\text{T}} + \beta _{8} {\text{GranCell}} \\ & \quad + \beta _{9} n{\text{RBC}} + \beta _{{10}} {\text{PC}}1 + \beta _{{11}} {\text{PC}}2 + \beta _{{12}} {\text{Type}} \\ & \quad + \varvec{\beta }_{{{\mathbf{13}}}} {\mathbf{Type*PFAS}} + [1|ID] \\ \end{aligned}\)
- \(5 - {\text{mC}}\;{\text{or}}\;5 - hmC\;{\text{Methylation}}\;{\text{at}}\;{\text{Sites}}\;{\text{with}}\;{\text{Type}} - {\text{Interaction}} \, = \, \beta _{0} + \varvec{\beta }_{{\mathbf{1}}} {\mathbf{PFAS}} + \beta _{2} {\text{Parity}} + \beta _{3} {\text{Smoking}} + \beta _{4} {\text{Race}} + \beta _{5} {\text{CD4T}} + \beta _{6} {\text{CD}}8{\text{T}} + \beta _{7} {\text{GranCell}} + \beta _{8} n{\text{RBC}} + \beta _{9} {\text{PC}}1 + \beta _{{10}} {\text{PC}}\)
- CD4T: CD4 T lymphocytes; CD8T: CD8 T lymphocytes; GranCell: granulated cells; MeFOSAA: 2-(N-methyl-perfluorooctane sulfonamido) acetic acid; nRBC: nucleated red blood cells; PC: principal component representing batch effects; PFAS: per-/polyfluoroalkyl substances; PFHxS: perfluorohexanesulphonic acid; PFDA: perfluorodecanoic acid; PFNA: perfluorononanoic acid; PFOA: perfluorooctanoic acid; PFOS: perfluorooctanesulfonic acid; PFUnDA: perfluoroundecanoic acid
