Fig. 2

Metagene profiling of 5hmC shows enrichment of 5hmC in gene bodies. Gene-body 5hmC content is associated with gene expression in HEK293T cells. A Metagene analysis using normalized coverage (left) and average number of 5hmCs at CpG sites (right) in HEK293T cells. Y-axis shows normalized reads coverage (left) and number of detected 5hmCs (right). B Heatmap showing the 5hmC distribution on individual genes in decreasing order of expression. The colors represent normalized reads coverage. C Heatmaps showing correlations with correlation coefficients between gene expression and gene-body 5hmC content in HEK293T cells. Heatmap was created using RPKM-normalized read counts within gene bodies. Normalization was performed using the RPKM method. Correlation coefficients were calculated by Pearson correlation