Fig. 2

Potential influence of AMA-induced local DNA methylation alteration. A Heatmap shows the scaled methylation level of each sample in hyper- or hypo-AMA-related DMR regions. B Column graph shows the percentage of AMA-related DMRs in different genetic elements. Hyper-DMR is on the top, while hypo-AMA-related DMR is on the bottom. C Pie charts show the proportion of hyper- or hypo-AMA-related DMRs located in or outside the ME regions. D Venn diagram presents the relationships among the nearest genes of AMA-related DMRs, genes in the longevity database, and genes recorded in the Aging Atlas database. Overlapping genes near AMA-related DMRs are shown in the frame. Genes associated with hyper-AMA-related DMRs are marked in red, while genes associated with hypo-DMRs are marked in blue. The bolded gene symbols refer to overlapping genes existing in both the longevity database and the Aging Atlas database. E Bubble chart shows representative KEGG pathways enriched for AMA-related DMR. The P value was determined by a hypergeometric test. F Bubble chart shows representative biological process Gene Ontology (GO) terms enriched for AMA-related DMR. The P value was determined using a hypergeometric test