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Fig. 3 | Clinical Epigenetics

Fig. 3

From: Azithromycin attenuates wheezing after pulmonary inflammation through inhibiting histone H3K27me3 hypermethylation mediated by EZH2

Fig. 3

AZM attenuates overexpression of P65, EZH2 and H3K27me3 and cytokines and alleviates wheezing time. A–C The expression levels of P65, EZH2 and H3K27me3 were examined by Western blotting (n = 4). D–G Effects of AZM on IL-2, IL-6, IL-10 and INF-γ in the BALF (n = 3). H Three shEZH2 intervention sites were used to optimize the transfection efficiency, and that of the lentiviral vector was analyzed by immunofluorescence containing EZH2 shRNA. Scale bars indicate 100 μm. I Lentiviral transfection efficiency of shEZH2 was analyzed by Western blotting. #P < 0.05 versus the shCtrl group (n = 3). J, K The expression of EZH2 and H3K27me3 after shEZH2 intervention was detected by protein immunoblotting. &P < 0.05 versus the Wheeze + shCtrl group (n = 3). L Effects of AZM on wheezing time in patients. M Effects of mycoplasma infection on AZM and wheezing time. *P < 0.05 versus the Wheeze group

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Clinical Epigenetics

ISSN: 1868-7083

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