Table 4 Methylation difference of HTRA1 between stroke cases and controls stratified by age in the prospective nested case–control study
CpG sites | Controls | Stroke cases | Crude OR (95% CI) | P value | OR (95% CI)☆ | P value☆ | OR (95% CI)* | P value* |
|---|---|---|---|---|---|---|---|---|
Median (IQR) | Median (IQR) | Per + 10% methylation | Per + 10% methylation | Per + 10% methylation | ||||
Participants < 70 years old (88 stroke cases vs. 90 controls) | ||||||||
HTRA1_A_CpG_1 | 0.07 (0.05–0.10) | 0.07 (0.05–0.09) | 0.72 (0.33–1.61) | 0.427 | 0.72 (0.33–1.61) | 0.428 | 0.67 (0.30–1.54) | 0.348 |
HTRA1_A_CpG_2 | 0.78 (0.74–0.84) | 0.77 (0.73–0.82) | 1.09 (0.85–1.40) | 0.504 | 1.08 (0.84–1.39) | 0.542 | 1.15 (0.88–1.50) | 0.316 |
HTRA1_A_CpG_3 | 0.44 (0.41–0.51) | 0.46 (0.41–0.52) | 1.02 (0.75–1.39) | 0.899 | 1.05 (0.76–1.44) | 0.772 | 1.05 (0.75–1.46) | 0.780 |
HTRA1_A_CpG_4 | 0.19 (0.16–0.21) | 0.20 (0.17–0.22) | 1.39 (0.65–2.99) | 0.400 | 1.38 (0.63–2.99) | 0.421 | 1.54 (0.69–3.43) | 0.295 |
HTRA1_A_CpG_5 | 0.41 (0.35–0.49) | 0.41 (0.35–0.46) | 0.89 (0.67–1.17) | 0.394 | 0.88 (0.66–1.16) | 0.365 | 0.87 (0.65–1.16) | 0.339 |
HTRA1_A_CpG_6 | 0.09 (0.08–0.11) | 0.08 (0.07–0.10) | 0.59 (0.30–1.18) | 0.135 | 0.57 (0.28–1.14) | 0.110 | 0.55 (0.27–1.12) | 0.101 |
HTRA1_A_CpG_7 | 0.39 (0.34–0.45) | 0.40 (0.35–0.45) | 1.20 (0.82–1.76) | 0.343 | 1.23 (0.84–1.81) | 0.297 | 1.22 (0.82–1.81) | 0.320 |
HTRA1_B_CpG_1 | 0.19 (0.11–0.29) | 0.18 (0.11–0.30) | 1.11 (0.91–1.36) | 0.299 | 1.11 (0.91–1.36) | 0.322 | 1.14 (0.93–1.41) | 0.208 |
HTRA1_B_CpG_2 | 0.12 (0.09–0.16) | 0.13 (0.08–0.17) | 1.17 (0.69–2.01) | 0.560 | 1.19 (0.69–2.04) | 0.534 | 1.19 (0.69–2.07) | 0.528 |
HTRA1_B_CpG_3.4 | 0.31 (0.26–0.35) | 0.33 (0.27–0.37) | 1.23 (0.85–1.79) | 0.278 | 1.21 (0.83–1.77) | 0.321 | 1.22 (0.83–1.79) | 0.312 |
HTRA1_B_CpG_5 | 0.34 (0.28–0.40) | 0.34 (0.26–0.43) | 1.06 (0.80–1.41) | 0.688 | 1.05 (0.79–1.39) | 0.751 | 1.04 (0.78–1.40) | 0.781 |
HTRA1_B_CpG_7 | 0.47 (0.39–0.53) | 0.46 (0.38–0.58) | 1.04 (0.83–1.32) | 0.720 | 1.03 (0.81–1.30) | 0.826 | 1.03 (0.80–1.31) | 0.833 |
HTRA1_B_CpG_8.9 | 0.31 (0.26–0.35) | 0.33 (0.27–0.37) | 1.23 (0.85–1.79) | 0.278 | 1.21 (0.83–1.77) | 0.321 | 1.22 (0.83–1.79) | 0.312 |
HTRA1_B_CpG_10 | 0.61 (0.55–0.69) | 0.66 (0.56–0.75) | 1.17 (0.91–1.51) | 0.233 | 1.17 (0.90–1.51) | 0.236 | 1.18 (0.90–1.54) | 0.224 |
HTRA1_B_CpG_11.12 | 0.75 (0.69–0.79) | 0.75 (0.70–0.82) | 1.14 (0.83–1.58) | 0.428 | 1.12 (0.81–1.55) | 0.509 | 1.11 (0.79–1.55) | 0.558 |
HTRA1_B_CpG_13.14.15 | 0.54 (0.46–0.61) | 0.56 (0.47–0.61) | 1.17 (0.88–1.54) | 0.281 | 1.15 (0.87–1.53) | 0.314 | 1.16 (0.87–1.54) | 0.317 |
HTRA1_B_CpG_16 | 0.48 (0.42–0.58) | 0.49 (0.37–0.57) | 0.92 (0.76–1.12) | 0.393 | 0.91 (0.75–1.11) | 0.351 | 0.90 (0.74–1.10) | 0.321 |
Participants ≥ 70 years old (51 stroke cases vs. 53 controls) | ||||||||
HTRA1_A_CpG_1 | 0.07 (0.06–0.08) | 0.07 (0.05–0.09) | 0.73 (0.25–2.13) | 0.563 | 0.66 (0.22–1.98) | 0.457 | 0.67 (0.20–2.22) | 0.511 |
HTRA1_A_CpG_2 | 0.76 (0.71–0.82) | 0.76 (0.71–0.81) | 0.86 (0.65–1.14) | 0.303 | 0.87 (0.65–1.16) | 0.330 | 0.86 (0.60–1.23) | 0.418 |
HTRA1_A_CpG_3 | 0.44 (0.38–0.47) | 0.46 (0.42–0.54) | 1.78 (1.08–2.93) | 0.024 | 1.77 (1.06–2.93) | 0.028 | 1.69 (0.97–2.93) | 0.063 |
HTRA1_A_CpG_4 | 0.20 (0.17–0.22) | 0.19 (0.17–0.21) | 1.02 (0.50–2.09) | 0.962 | 1.01 (0.49–2.10) | 0.975 | 0.90 (0.40–2.02) | 0.803 |
HTRA1_A_CpG_5 | 0.44 (0.38–0.51) | 0.40 (0.34–0.45) | 0.71 (0.50–1.01) | 0.054 | 0.72 (0.50–1.02) | 0.065 | 0.62 (0.40–1.01) | 0.053 |
HTRA1_A_CpG_6 | 0.09 (0.08–0.10) | 0.08 (0.07–0.10) | 0.94 (0.35–2.48) | 0.895 | 1.02 (0.38–2.74) | 0.973 | 0.99 (0.33–2.96) | 0.988 |
HTRA1_A_CpG_7 | 0.39 (0.35–0.42) | 0.40 (0.34–0.48) | 1.10 (0.69–1.75) | 0.688 | 1.06 (0.66–1.71) | 0.806 | 0.91 (0.54–1.52) | 0.711 |
HTRA1_B_CpG_1 | 0.17 (0.12–0.25) | 0.18 (0.10–0.29) | 1.08 (0.85–1.36) | 0.530 | 1.06 (0.84–1.35) | 0.621 | 0.98 (0.75–1.28) | 0.900 |
HTRA1_B_CpG_2 | 0.13 (0.09–0.17) | 0.11 (0.08–0.16) | 0.94 (0.52–1.69) | 0.832 | 0.98 (0.54–1.77) | 0.936 | 1.18 (0.60–2.31) | 0.636 |
HTRA1_B_CpG_3.4 | 0.30 (0.25–0.35) | 0.32 (0.27–0.37) | 1.72 (0.97–3.06) | 0.064 | 1.73 (0.97–3.08) | 0.064 | 2.36 (1.18–4.73) | 0.015 |
HTRA1_B_CpG_5 | 0.31 (0.26–0.39) | 0.38 (0.28–0.47) | 1.50 (1.03–2.19) | 0.033 | 1.59 (1.07–2.37) | 0.023 | 1.70 (1.08–2.67) | 0.021 |
HTRA1_B_CpG_7 | 0.49 (0.40–0.53) | 0.48 (0.41–0.57) | 1.10 (0.82–1.48) | 0.540 | 1.09 (0.81–1.47) | 0.583 | 1.09 (0.78–1.52) | 0.614 |
HTRA1_B_CpG_8.9 | 0.30 (0.25–0.35) | 0.32 (0.27–0.37) | 1.72 (0.97–3.06) | 0.064 | 1.73 (0.97–3.08) | 0.064 | 2.36 (1.18–4.73) | 0.015 |
HTRA1_B_CpG_10 | 0.64 (0.54–0.69) | 0.68 (0.53–0.76) | 1.14 (0.87–1.49) | 0.333 | 1.14 (0.87–1.49) | 0.338 | 1.23 (0.91–1.65) | 0.178 |
HTRA1_B_CpG_11.12 | 0.73 (0.68–0.78) | 0.77 (0.68–0.83) | 1.54 (1.01–2.35) | 0.043 | 1.49 (0.98–2.28) | 0.064 | 1.35 (0.85–2.15) | 0.203 |
HTRA1_B_CpG_13.14.15 | 0.53 (0.47–0.57) | 0.57 (0.50–0.67) | 1.76 (1.16–2.69) | 0.008 | 1.78 (1.15–2.76) | 0.009 | 1.76 (1.09–2.86) | 0.021 |
HTRA1_B_CpG_16 | 0.47 (0.39–0.55) | 0.49 (0.37–0.60) | 1.02 (0.79–1.30) | 0.901 | 1.03 (0.80–1.32) | 0.837 | 1.04 (0.79–1.37) | 0.763 |