Table 3 Methylation difference of HTRA1 between stroke cases and controls stratified by age in the case–control study
CpG sites | Controls | Stroke cases | Crude OR (95% CI) | P value | OR (95% CI)☆ | P value☆ | OR (95% CI)* | P value* |
|---|---|---|---|---|---|---|---|---|
Median (IQR) | Median (IQR) | Per + 10% methylation | Per + 10% methylation | Per + 10% methylation | ||||
Participants < 70 years old (116 stroke cases vs. 125 controls) | ||||||||
HTRA1_A_CpG_1 | 0.09 (0.05–0.13) | 0.11 (0.08–0.13) | 1.23 (0.84–1.81) | 0.293 | 1.23 (0.84–1.81) | 0.293 | 1.20 (0.76–1.89) | 0.448 |
HTRA1_A_CpG_2 | 0.65 (0.57–0.79) | 0.64 (0.56–0.74) | 0.98 (0.86–1.11) | 0.707 | 0.98 (0.86–1.11) | 0.696 | 0.96 (0.83–1.10) | 0.510 |
HTRA1_A_CpG_3 | 0.28 (0.22–0.35) | 0.30 (0.25–0.35) | 1.10 (0.85–1.41) | 0.484 | 1.10 (0.85–1.41) | 0.489 | 1.13 (0.85–1.51) | 0.409 |
HTRA1_A_CpG_4 | 0.12 (0.09–0.15) | 0.13 (0.11–0.15) | 1.31 (0.74–2.30) | 0.354 | 1.30 (0.74–2.29) | 0.369 | 1.02 (0.54–1.94) | 0.951 |
HTRA1_A_CpG_5 | 0.37 (0.31–0.43) | 0.38 (0.30–0.43) | 0.96 (0.81–1.15) | 0.679 | 0.96 (0.81–1.15) | 0.680 | 0.97 (0.79–1.18) | 0.731 |
HTRA1_A_CpG_6 | 0.12 (0.10–0.14) | 0.10 (0.06–0.14) | 0.66 (0.40–1.08) | 0.097 | 0.66 (0.40–1.09) | 0.106 | 0.69 (0.41–1.16) | 0.163 |
HTRA1_A_CpG_7 | 0.36 (0.28–0.45) | 0.37 (0.31–0.45) | 1.04 (0.86–1.27) | 0.685 | 1.04 (0.85–1.27) | 0.713 | 1.06 (0.85–1.34) | 0.602 |
HTRA1_B_CpG_1 | 0.16 (0.09–0.40) | 0.18 (0.11–0.50) | 1.09 (0.98–1.21) | 0.122 | 1.09 (0.98–1.22) | 0.123 | 1.11 (0.98–1.25) | 0.107 |
HTRA1_B_CpG_2 | 0.09 (0.05–0.12) | 0.11 (0.07–0.15) | 1.66 (0.93–3.03) | 0.102 | 1.65 (0.92–3.01) | 0.103 | 1.80 (0.96–3.23) | 0.062 |
HTRA1_B_CpG_3.4 | 0.28 (0.23–0.32) | 0.29 (0.25–0.34) | 1.41 (0.97–2.03) | 0.069 | 1.42 (0.98–2.05) | 0.063 | 1.29 (0.85–1.96) | 0.230 |
HTRA1_B_CpG_5 | 0.25 (0.19–0.33) | 0.28 (0.22–0.33) | 1.19 (0.92–1.54) | 0.190 | 1.20 (0.93–1.56) | 0.169 | 1.18 (0.88–1.58) | 0.274 |
HTRA1_B_CpG_7 | 0.47 (0.35–0.54) | 0.46 (0.38–0.53) | 1.07 (0.88–1.29) | 0.520 | 1.06 (0.88–1.29) | 0.535 | 0.99 (0.79–1.23) | 0.906 |
HTRA1_B_CpG_8.9 | 0.28 (0.23–0.32) | 0.29 (0.25–0.34) | 1.41 (0.97–2.03) | 0.069 | 1.42 (0.98–2.05) | 0.063 | 1.29 (0.85–1.96) | 0.230 |
HTRA1_B_CpG_10 | 0.61 (0.53–0.71) | 0.64 (0.55–0.70) | 1.14 (0.94–1.38) | 0.180 | 1.14 (0.94–1.38) | 0.173 | 1.13 (0.92–1.40) | 0.249 |
HTRA1_B_CpG_11.12 | 0.72 (0.64–0.79) | 0.73 (0.67–0.80) | 1.13 (0.89–1.43) | 0.330 | 1.12 (0.89–1.42) | 0.343 | 1.11 (0.85–1.45) | 0.451 |
HTRA1_B_CpG_13.14.15 | 0.48 (0.40–0.56) | 0.50 (0.45–0.57) | 1.24 (0.99–1.55) | 0.066 | 1.24 (0.99–1.55) | 0.068 | 1.19 (0.92–1.55) | 0.186 |
HTRA1_B_CpG_16 | 0.51 (0.43–0.62) | 0.53 (0.44–0.66) | 1.08 (0.92–1.27) | 0.367 | 1.08 (0.92–1.28) | 0.356 | 1.03 (0.85–1.24) | 0.800 |
Participants ≥ 70 years old (74 stroke cases vs. 65 controls) | ||||||||
HTRA1_A_CpG_1 | 0.09 (0.06–0.12) | 0.10 (0.07–0.14) | 1.38 (0.77–2.45) | 0.278 | 1.37 (0.77–2.44) | 0.282 | 1.43 (0.73–2.82) | 0.300 |
HTRA1_A_CpG_2 | 0.63 (0.56–0.71) | 0.65 (0.59–0.78) | 1.08 (0.92–1.27) | 0.335 | 1.09 (0.92–1.28) | 0.340 | 1.05 (0.85–1.30) | 0.631 |
HTRA1_A_CpG_3 | 0.32 (0.25–0.38) | 0.28 (0.22–0.36) | 0.76 (0.54–1.05) | 0.097 | 0.74 (0.53–1.04) | 0.087 | 0.63 (0.42–0.94) | 0.022 |
HTRA1_A_CpG_4 | 0.12 (0.09–0.15) | 0.12 (0.11–0.15) | 1.79 (0.87–3.71) | 0.116 | 1.80 (0.87–3.74) | 0.113 | 1.92 (0.78–4.70) | 0.156 |
HTRA1_A_CpG_5 | 0.37 (0.3–0.46) | 0.38 (0.29–0.49) | 1.04 (0.82–1.32) | 0.750 | 1.04 (0.82–1.32) | 0.752 | 1.01 (0.76–1.33) | 0.967 |
HTRA1_A_CpG_6 | 0.12 (0.10–0.15) | 0.10 (0.07–0.13) | 0.27 (0.12–0.63) | 0.003 | 0.27 (0.11–0.62) | 0.002 | 0.29 (0.12–0.73) | 0.008 |
HTRA1_A_CpG_7 | 0.36 (0.27–0.46) | 0.38 (0.30–0.48) | 1.16 (0.88–1.53) | 0.282 | 1.15 (0.88–1.52) | 0.310 | 1.12 (0.81–1.53) | 0.497 |
HTRA1_B_CpG_1 | 0.15 (0.09–0.42) | 0.17 (0.09–0.42) | 0.96 (0.83–1.10) | 0.557 | 0.95 (0.83–1.10) | 0.529 | 0.94 (0.79–1.13) | 0.511 |
HTRA1_B_CpG_2 | 0.08 (0.05–0.14) | 0.09 (0.07–0.12) | 0.92 (0.56–1.52) | 0.756 | 0.92 (0.56–1.52) | 0.749 | 0.70 (0.39–1.28) | 0.251 |
HTRA1_B_CpG_3.4 | 0.30 (0.25–0.34) | 0.29 (0.23–0.34) | 0.92 (0.60–1.41) | 0.701 | 0.93 (0.60–1.44) | 0.737 | 0.85 (0.50–1.43) | 0.534 |
HTRA1_B_CpG_5 | 0.27 (0.22–0.36) | 0.28 (0.21–0.34) | 0.96 (0.71–1.30) | 0.779 | 0.97 (0.71–1.32) | 0.822 | 0.87 (0.60–1.26) | 0.455 |
HTRA1_B_CpG_7 | 0.48 (0.38–0.55) | 0.49 (0.34–0.56) | 1.04 (0.84–1.29) | 0.731 | 1.05 (0.84–1.32) | 0.650 | 1.00 (0.75–1.33) | 0.999 |
HTRA1_B_CpG_8.9 | 0.30 (0.25–0.34) | 0.29 (0.23–0.34) | 0.92 (0.60–1.41) | 0.701 | 0.93 (0.60–1.44) | 0.737 | 0.85 (0.50–1.43) | 0.534 |
HTRA1_B_CpG_10 | 0.63 (0.55–0.73) | 0.62 (0.53–0.72) | 0.94 (0.74–1.19) | 0.615 | 0.94 (0.74–1.20) | 0.619 | 0.78 (0.57–1.07) | 0.117 |
HTRA1_B_CpG_11.12 | 0.72 (0.66–0.82) | 0.72 (0.65–0.82) | 1.06 (0.81–1.37) | 0.685 | 1.06 (0.81–1.39) | 0.669 | 0.96 (0.67–1.37) | 0.815 |
HTRA1_B_CpG_13.14.15 | 0.51 (0.42–0.62) | 0.51 (0.44–0.57) | 1.02 (0.79–1.32) | 0.863 | 1.03 (0.79–1.34) | 0.819 | 0.98 (0.70–1.38) | 0.914 |
HTRA1_B_CpG_16 | 0.55 (0.47–0.67) | 0.53 (0.45–0.69) | 0.95 (0.78–1.17) | 0.642 | 0.96 (0.78–1.17) | 0.659 | 0.92 (0.72–1.17) | 0.496 |