Fig. 6

Altered sperm DNA methylation detected months following chemotherapy treatments. A RLGS and B 450 K array analysis of DNA methylation over time/post-chemotherapy treatment of CC patients (left) and from HD and TC patients (right). The dotted lines drawn within each graph indicate the average number of sites altered in CC patients for each technique used. C MCC-Seq (5-methylcytosine capture sequencing) analysis showing the number of CpG sites that demonstrated > 20% methylation difference within individual patients, comparing the 12 and 18 months post-treatment, with the number of dynamic CpG sites indicated. D The total number of sites altered is expressed as a percentage of all the sites (1,711,875) analyzed. ***p < 0.0001, chi-squared test with Yate’s correction