Fig. 3

Mechanism of decreased SUV39H1 regulating CREMα expression in CD4⁺ T cells from 3 normal controls. a, b Relative SUV39H1 and CREMα protein expressions in normal CD4⁺ T cells transfected with SUV39H1-siRNA or control-siRNA were quantified by western blot analysis 72 h after transfection. β-actin was used as endogenous control. c, d Relative SUV39H1 (c) and H3K9me3 (d) enrichments at the CREMα promoter in normal CD4⁺ T cells transfected with SUV39H1-siRNA or control-siRNA were analyzed by ChIP combined with qPCR 72 h after transfection. Input DNA was used as endogenous control, and IgG was used as negative control. e, f, g, h Relative levels of DNA methylation (e), DNMT3a (f), H3K4me3 (g), and Set1 (h) at the CREMα promoter in normal CD4⁺ T cells transfected with SUV39H1-siRNA or control-siRNA were evaluated by ChIP or MeDIP combined with qPCR 72 h after transfection. Input DNA was used as endogenous control, and IgG was used as negative control. All experiments were repeated three times