Fig. 1

CREMα expression and H3K9me3 enrichment at the CREMα promoter in CD4⁺ T cells. a Relative CREMα mRNA level in normal and SLE CD4⁺ T cells was evaluated by qPCR and normalized to β-actin. b Relative H3K9me3 enrichment at the CREMα promoter in normal and SLE CD4⁺ T cells was measured by ChIP combined with qPCR. Input DNA was used as endogenous control, and IgG was used as negative control. c The correlation between H3K9me3 enrichment at the CREMα promoter and CREMα mRNA level in SLE CD4⁺ T cells. d The correlation between H3K9me3 enrichment at the CREMα promoter in SLE CD4⁺ T cells and SLEDAI. All reactions were run three times