Fig. 2

Deletion of Eed in oocytes reduced H3K27me3 in oocytes of primary and secondary follicles. a Representative images of H3K27me3 (red) immunostaining analysis in primordial (top), primary (middle) and secondary (bottom) follicle oocytes from Eed-wt and Eed-hom females. White arrowheads indicate the oocyte nucleus as defined by Lamin B1 (green). DAPI (blue) shows DNA in somatic cells. Images are representative of two ovaries from three biological replicates. Scale bars: 20 μm. b–c Quantification of H3K27me3 within oocyte nuclei of primary (b) and secondary (c) follicles from Eed-wt and Eed-hom females. Average intensity of Eed-wt was set to 1.0. **P < 0.005, two-tailed Mann–Whitney U test, N = 63 Eed-wt and 67 Eed-hom primary follicle oocytes. ****P < 0.0001, two-tailed Mann–Whitney U test, N = 45 Eed-wt and 47 Eed-hom secondary follicle oocytes. Error bars represent mean ± standard deviation