Fig. 1
From: Transient Polycomb activity represses developmental genes in growing oocytes
PRC2 acts transiently within primary and secondary follicle growing oocytes. (a-d) Representative images of EZH2, SUZ12 and EED (red) IF analysis in (a) primordial, primary, secondary and antral follicles. (b) Comparison of EED (red, left panels) versus DNMT3L (red, right panels) IF analysis in primary, secondary and antral follicles. (c) Surrounded nucleolus (SN) GV oocytes. (d) MII oocytes. α-Tubulin (green) identifies meiotic spindles. M: metaphase plate, PB: polar body. (e) Zygotes 12 h after fertilisation for ≥ 10 zygotes imaged per antibody combination. M: maternal pronucleus, P: paternal pronucleus, PB: polar body. In a-c white arrowheads indicate the oocyte nucleus defined by Lamin B1 (green) and DAPI (blue) shows DNA. In c-e images represent compressed z-stack images of whole-mount oocytes or zygotes. Scale bars: 20 μm. Images in a and b are representative of two ovaries from three separate females and in c-e images are representative of ≥ 10 oocytes per antibody combination