Fig. 2

SNP-related CpGs identified at screening. This Manhattan plot shows identified SNP-related CpG sites in the screening adolescent cohort. This figure comprises results for all investigated SNP sites. All four studied SNPs were tested independently. Analyses were conducted with the R package “limma,” the dominant model was used. The smallest p value for every SNP–CpG association is included in the image. Raw p values from limma are visualized. The dashed red line shows a threshold for significance after correction for multiple testing (false discovery rate) and the number of SNP sites. Only probes that passed initial QC were included in the analysis. CpG sites located at the MAD1L1 gene are highlighted in yellow. All statistically significant results are annotated by name. SNP, single nucleotide polymorphism; QC, quality control