Fig. 5
From: Epigenetic silencing of JAM3 promotes esophageal cancer development by activating Wnt signaling
The effect of JAM3 on Wnt/β-catenin signaling in EC. a Western blot shows the levels of JAM3, β-catenin, p-β-catenin, Myc and cyclinD1 in EC cells. Actin: internal control. Scrambled: siRNA negative control; siRNA#2: siRNA targeting JAM3. b, c Representative tumors from JAM3 unexpressed and re-expressed KYSE410 cell xenografts in mice. d Growth curves of JAM3 unexpressed and re-expressed KYSE410 cell tumors. ***p < 0.001. e Tumor weight in JAM3 unexpressed and re-expressed KYSE410 cell xenografts. Bars represent the mean of tumor weight. ***p < 0.001. f Representative results of immunohistochemistry for JAM3, β-catenin and p-β-catenin in xenografts