Fig. 3
From: Epigenetic silencing of JAM3 promotes esophageal cancer development by activating Wnt signaling
The effect of JAM3 on cell proliferation, invasion and migration. a Growth curves represent the effects of JAM3 in EC cells. b Colony formation results show that the colony number was reduced by re-expression of JAM3 in KYSE30 and KYSE410 cells, and increased by knockdown of JAM3 in YES2 and KYSE450 cells. The average number of tumor clones is represented by the bar diagram. c Cell migration experiment in KYSE30 and KYSE410 cells before and after re-expression of JAM3, and in YES2 and KYSE450 cells before and after knockdown of JAM3. The average number of migration cells is presented by a bar diagram. d Cell invasion experiment in KYSE30 and KYSE410 cells before and after re-expression of JAM3, and in YES2 and KYSE450 cells before and after knockdown of JAM3. The average number of invasion cells is presented by a bar diagram. Each experiment was repeated in triplicate. *p < 0.05. **p < 0.01. ***p < 0.001. e Western blots show the effects of knockdown of JAM3 by different siRNA. Scrambled: siRNA negative control; siRNA#1 and siRNA#2: siRNA for JAM3. f The levels of JAM3, MMP2 and MMP9 were detected by western blot. Actin: internal control. Scrambled: siRNA negative control; siRNA#2: siRNA targeting JAM3