Fig. 2
From: Epigenetic silencing of JAM3 promotes esophageal cancer development by activating Wnt signaling
The methylation and expression status of JAM3 in EC samples. a Representative MSP results of JAM3 in normal esophageal mucosa and primary EC samples. NE: normal esophageal mucosa; EIN: esophageal intraepithelial neoplasia; EC: esophageal cancer samples. IVD: in vitro methylated DNA, serves as methylation control; NL: normal peripheral lymphocytes DNA, serves as unmethylation control; and H2O: double distilled water. b The frequency of JAM3 methylation in EIN and EC. *** p < 0.001. c The association of JAM3 methylation and OS of EC. d JAM3 staining in EC and adjacent tissue samples (top: 200 × ; bottom: 400 ×). e Box plots for JAM3 expression; horizontal lines represent the median score; the bottom and top of the boxes represent the 25th and 75th percentiles, respectively; vertical bars represent expression levels. **p < 0.01. f Bar diagram: the levels of JAM3 expression and methylation status. *p < 0.05