Quantification of DNA methylation for carcinogenic risk estimation in patients with non-alcoholic steatohepatitis

Kuramoto, Junko; Arai, Eri; Fujimoto, Mao; Tian, Ying; Yamada, Yuriko; Yotani, Takuya; Makiuchi, Satomi; Tsuda, Noboru; Ojima, Hidenori; Fukai, Moto; Seki, Yosuke; Kasama, Kazunori; Funahashi, Nobuaki; Udagawa, Haruhide; Nammo, Takao; Yasuda, Kazuki; Taketomi, Akinobu; Kanto, Tatsuya; Kanai, Yae

doi:10.1186/s13148-022-01379-4

Clinical Epigenetics

Table 3 Multivariate analysis using the 91 samples of non-cancerous liver tissue showing non-alcoholic steatohepatitis (NASH) derived from biopsy specimens of patients with morbid obesity but without hepatocellular carcinoma (HCC) (NASH-O samples) and the 22 samples of non-cancerous liver tissue showing NASH derived from partial hepatectomy specimens of patients with HCC (NASH-W samples) in the initial cohorts

From: Quantification of DNA methylation for carcinogenic risk estimation in patients with non-alcoholic steatohepatitis

Probe ID^a	Variables	Odds ratio^b	95% confidence interval	P value^c
cg11820154	Ballooning^d	0.460	0.031–6.765	0.571
	Brunt classification^e	0.347	0.100–1.207	0.096
	DNA methylation quantification	1138.492	52.740–24,576.521	7.13 × 10⁻⁶
cg26583598	Ballooning^d	0.692	0.051–9.460	0.782
	Brunt classification^e	0.286	0.090–0.915	0.035
	DNA methylation quantification	1035.707	54.365–19,731.273	3.89 × 10⁻⁶
cg18210511	Ballooning^d	8.990	0.219–369.308	0.247
	Brunt classification^e	0.482	0.207–1.123	0.091
	DNA methylation quantification	248.725	30.517–2027.198	2.56 × 10⁻⁷
cg00431813	Ballooning^d	5.786	0.132–252.827	0.362
	Brunt classification^e	0.686	0.275–1.714	0.420
	DNA methylation quantification	232.059	31.166–1727.915	1.05 × 10⁻⁷
cg19861632	Ballooning^d	2.047	0.191–21.929	0.554
	Brunt classification^e	0.610	0.302–1.232	0.168
	DNA methylation quantification	207.072	20.883–2053.237	5.20 × 10⁻⁶
cg05861743	Ballooning^d	1.491	0.133–16.687	0.746
	Brunt classification^e	0.503	0.229–1.106	0.088
	DNA methylation quantification	190.885	24.574–1482.750	5.14 × 10⁻⁷
cg09580822	Ballooning^d	1.491	0.124–15.383	0.795
	Brunt classification^e	0.503	0.248–1.235	0.148
	DNA methylation quantification	190.885	23.957–1361.603	4.61 × 10⁻⁷
cg09580859	Ballooning^d	9.892	0.299–327.815	0.200
	Brunt classification^e	0.506	0.226–1.133	0.098
	DNA methylation quantification	180.393	24.567–1324.612	3.27 × 10⁻⁷
cg14950303	Ballooning^d	1.256	0.114–13.824	0.853
	Brunt classification^e	0.702	0.315–1.565	0.387
	DNA methylation quantification	144.050	21.556–962.621	2.92 × 10⁻⁷
cg26385097	Ballooning^d	1.577	0.145–17.140	0.708
	Brunt classification^e	0.595	0.275–1.288	0.188
	DNA methylation quantification	141.119	20.394–976.513	5.30 × 10⁻⁷

^aProbe ID of the Infinium HumanMethylation450 BeadChip
^bTop 10 CpG sites showing the highest odds ratio of being NASH-W samples are summarized in this table. The results of multivariate analysis for all 21 CpG sites are shown in Additional file 1: Table S7
^cP values less than 0.05 are underlined
^dBallooning was evaluated according to the non-alcoholic fatty disease activity score (Ref. 26)
^eFibrosis stage was defined according to the Brunt classification (Ref. 27)

Back to article page

ISSN: 1868-7083

Contact us

Submission enquiries: avalyn.villar@springernature.com
General enquiries: info@biomedcentral.com