Fig. 2

Reduced cell viability associated with induced PRELP gene expression in bladder cancer cells. PRELP gene expression was induced by the addition of doxycycline (DOX, 1 μg/ml) in the lentiviral expression system. Expression of myc-tagged PRELP protein was analyzed using whole-cell extracts from RT4 (A) and J82 (B) cells with or without DOX. The left side indicated the protein size marker. Alpha-tubulin; loading control. Cell viability of RT4 (C) and J82 (D) cells was evaluated using cell counting kit (CCK)-8 assays. Black lines; without DOX, Red lines; with DOX. Error bars indicate biological replicates (n = 3). Statistical analysis was performed using paired Student’s t test. *P < 0.05, ****P < 0.0001