Fig. 2

Characterization of PCDHB15-overexpressing clones. A Western blot analysis of endogenous PCDHB15 in WM115 and WM266-4 cells and B of the overexpression of the PCDHB15 construct in WM266-4 cells, mock (transfected with the empty pCMV6 vector), clone 8, 12 and 13, revealed by the antibody against PCDHB15 or against DDK (for the constructs only). The Western blot is representative of n = 3. Beta-actin was used as loading control (bottom). C PCDHB15 mRNA quantification by RT-qPCR (on n = 3 biologically independent experiments, ANOVA test, *: p < 0.05; **:p< 0.01. ***: p < 0.001). The value in WM266-4 cells was considered as 1. D Cell surface expression of PCDHB15 measured by immunolabeling and flow cytometry. Black and white histograms display the cell surface fluorescence associated with PCDHB15 and isotypic control labeling, respectively