Table 4 Bronchoalveolar lavage fluid, bronchial aspirates, and bronchial washings DNA methylation-based liquid biopsy for LC
Purpose | Genes | Cases | Controls | Sensitivity/specificity | Samples | Methods | References |
|---|---|---|---|---|---|---|---|
Diagnosis | RASSF1A/SHOX2 | 123 | 130 | 71.5/90.0% | BALF | RT-PCR | [79] |
Diagnosis | RASSF1A/SHOX2 | 284 | 38 | 81.0/97.4% | BALF | RT-PCR | [76] |
Diagnosis | CDH1/AFR/GSTP1/p16/RAR-β2/MGMT/RASSF1A/APC | 31 | 10 | –/– | BALF | RT-PCR and RT-MSP | [80] |
Diagnosis | SHOX2 | 281 | 242 | 68.0/95.0% | bronchial aspirates | RT-PCR | [81] |
Diagnosis | PHF11/PDGFRA/TFAP2A/PRR15/HOXA11/TOX2/TBX15 | 70 | 53 | 87.0/83.3% | bronchial washings | 450Â K Methylation BeadChip and Pyrosequencing | [82] |