Fig. 3
RCC cell proliferation is suppressed, and T cell activation is enhanced by silencing HDAC3 via miR-195-5p. A miRNAs targeted by HDAC3 determined with TransmiR v2.0 website. B Venn diagram of Intersection of miRNAs targeted by HDAC3 predicted with TransmiR v2.0 and RNAInter. C Venn diagram of RCC-related miRNAs and candidate targeted miRNAs in the GeneCards database. D miR-195-5p expression in RCC and adjacent non-cancerous tissues determined by RT-qPCR (n = 58). *p < 0.05 versus the adjacent non-cancerous tissues. E miR-195-5p expression in RCC cell line (A498) and normal renal cell line (HK-2) determined by RT-qPCR. *p < 0.05 versus the HK-2 cell line. F The expression of HDAC3 in A498 cells normalized to GAPDH determined by Western blot analysis. *p < 0.05 versus the vector or si-NC group. G miR-195-5p expression in A498 cells after overexpression or silencing of HDAC3 determined by RT-qPCR. *p < 0.05 versus the vector or si-NC group. H The expression of HDAC3 in A498 cells normalized to GAPDH determined by Western blot analysis and miR-195-5p expression determined by RT-qPCR after transfection. *p < 0.05 between the two groups. I A498 cell proliferation detected by EdU assay. J IFN-γ+ T cell proportion and CD3+ T cell proliferation detected by flow cytometry. *p < 0.05 between the two groups. K The expression of IFN-γ released from CD3+T cells determined by ELISA. *p < 0.05 between the two groups. The results were measurement data and expressed as mean ± standard deviation. The results were collected from at least 3 independent cell experiments. Data between RCC and adjacent non-cancerous tissues were analyzed by paired t test, and data comparison between the other two groups was analyzed by unpaired t test