Fig. 1

PDCD5 is under-expressed in RCC tissues and cells. A The expression of PDCD5 in RCC and adjacent non-cancerous tissues determined by Western blot analysis (n = 58). *p < 0.05 versus the adjacent non-cancerous tissues. B The expression of PDCD5 in RCC cell lines (Caki-1, A498, 786-O, and 769-P) and normal renal cell line (HK-2) normalized to GAPDH determined by Western blot analysis. C The expression of PDCD5 in RCC cells normalized to GAPDH determined by Western blot analysis to confirm the transfection efficiency. *p < 0.05 versus the vector-treated cells. D A498 cell proliferation following PDCD5 overexpression detected by EdU assay. E IFN-γ⁺ T cell proportion and CD3⁺ T cell proliferation following PDCD5 overexpression detected by flow cytometry. F The expression of IFN-γ released from CD3⁺ T cells following PDCD5 overexpression determined by ELISA. *p < 0.05 between the two groups. The results were measurement data and expressed as mean ± standard deviation. The results were collected from at least 3 independent cell experiments