Effect of menopausal hormone therapy on methylation levels in early and late postmenopausal women

Clinical Epigenetics

Table 2 Methylation at previously identified CpGs most strongly affected by smoking

CpG	^aChr:pos	Nearest gene	Baseline		36-month visit
CpG	^aChr:pos	Nearest gene	Effect (SE)	^bp-value	Effect (SE)	^bp-value
cg09935388	1:92947588	GFI1	− 0.094 (0.063)	0.14	− 0.063 (0.057)	0.27
cg19859270	3:98251294	GPR15	− 0.078 (0.023)	9.8 × 10^–4	− 0.045 (0.023)	0.047
cg23576855	5:373299	AHRR	− 0.24 (0.12)	0.04	− 0.25 (0.12)	0.04
cg05575921	5:373378	AHRR	− 0.46 (0.07)	3.9 × 10^–10	− 0.44 (0.072)	4.0 × 10^–9
cg03636183	19:17000585	F2RL3	− 0.13 (0.033)	2.4 × 10^–4	− 0.12 (0.030)	1.4 × 10^–4

Data are shown as the effect of smoking on methylation (M-values) at the baseline visit and 36 months after treatment across all treatment groups
^aChromosome and position (base pair) are based on build 37 (hg19) of the human genome reference sequence
^bp-values were derived from linear regression analyses for smoking status (never, former, or current; coded as 0, 1, 2) with adjustment for age, ethnicity, time-since-menopause, randomized treatment, and estimated blood cell fractions

ISSN: 1868-7083