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Fig. 1 | Clinical Epigenetics

Fig. 1

From: Genome-wide promoter methylation profiling in a cellular model of melanoma progression reveals markers of malignancy and metastasis that predict melanoma survival

Fig. 1

Distinct methylation patterns characterize cell lines representing melanoma progression. A. Global DNA methylation content analyzed by HPLC shows the percentage of 5-methylcytosine in the genome for two biological replicates from the cell lines melan-a, 4C, 4C11−, 4C11+. B. Principal component analysis of DNA methylation data from three biological samples of the melan-a, 4C, 4C11− and 4C11+ cell lines shows close values of Principal Components 1 and 2 for samples from the same cell line. C. A heatmap represented with light yellow for regions with low methylation and dark blue for regions with high methylation shows the top most variant regions of 1,000 bp for each sample. Unsupervised hierarchical clustering in the upper part of the heatmap groups 4C and 4C11− in a common branch closer to 4C11 + than melan-a melanocytes. melan-a: non-tumorigenic melanocyte lineage; 4C: premalignant melanocyte lineage; 4C11−: non-metastatic melanoma cell line; 4C11 + : metastatic melanoma cell line

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Clinical Epigenetics

ISSN: 1868-7083

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