Table 3 Primer sets used for pyrosequencing and transcriptional analysis used in this study, including RT-qPCR
Application | Name | Oligo sequence (5′–3′) |
|---|---|---|
Pyrosequencing | pyro_MIR4520A_FW | GTTTAAATTTTTTTTTGATTTGGATAGAAA |
pyro_MIR4520A_RV | AAAACATACCCTCAATTCCAAAAAAAT C | |
pyro_MIR4520A_Seq | TTTTTTTTGATTTGGATAGAAAATA | |
pyro_PRKAR1b/HEATR2_Promoter_FW | TTTAGGGGTAGGTTTAGGTTTATAGT | |
pyro_PRKAR1b/HEATR2_Promoter_RV | CCAACCTACCTACTAAACCTTATC | |
pyro_PRKAR1b/HEATR2_Promoter_Seq | GGTAGGTTTAGGTTTATAGTT | |
pyro_PRKAR1b_GB_FW | GGTTTTATAGGGATGAGGTTTTATGTATAG | |
pyro_PRKAR1b_GB_RV | ACCACCCACAACATCAAACAAA | |
pyro_PRKAR1b_GB_Seq | AGGTTTTATGTATAGGGTAT | |
pyro_HEATR2_GB_FW | GGTAGGGAGGTATTTGGTTTAAGA | |
pyro_HEATR2_GB_RV | TAAAACCCCCCCACCTACCCTTACC | |
pyro_HEATR2_GB_Seq | GGGAGGTATTTGGTTTAAGATT | |
pyro_DUSP22_FW | GTGAGGTTTGAGGTTAGAGATTTAG | |
pyro_DUSP22_RV | ATCTCCAAATCCCCCTTTAACC | |
pyro_DUSP22_Seq | TTTAGGGTAGGGAGG | |
pyro_CES1_FW | GGTTGGTCGGGTTTAGTTGTTTTAAG | |
pyro_CES1_RV | AAACAACACAATCCCTCTAAACTAC | |
pyro_CES1_Seq | TTAAGTTTAAGTTTTAATATGGAA | |
RT-qPCR | qPCR_PRKAR1b_GB_FW | ACAGGGGAGTGAGGTGAGTG |
qPCR_PRKAR1b_GB_RV | TCTTTGAGGACCTGCTGGAT | |
qPCR_HEATR2_FW | AGCCCTGGCGTCGTGATTAGT | |
qPCR_HEATR2_RV | CCCGTTGAGCACACAGAGGCCTA | |
qPCR_DUSP22_FW | CGCTGACTTGTTGACACTGC | |
qPCR_DUSP22_RV | GCTCAATTGTTCCGCGTCTC | |
qPCR_CES1_FW | TTGCTGCCCATGAAAACGTG | |
qPCR_CES1_RV | GCATTGGAATCAACCAGCC | |
HPRT_FW | AGCCCTGGCGTCGTGATTAGT | |
HPRT_RV | CCCGTTGAGCACACAGAGGCCTA |