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Fig. 5 | Clinical Epigenetics

Fig. 5

From: LINC00114 stimulates growth and glycolysis of esophageal cancer cells by recruiting EZH2 to enhance H3K27me3 of DLC1

Fig. 5

Knocked down DLC1 mitigates the role of suppressed LINC00114 in growth and glycolysis of EC cells. A, B RT-qPCR and Western blot detected DLC1 expression in Eca109 cells after co-transfection with sh-LINC00114 and si-DLC1; C, D RT-qPCR and Western blot detected DLC1 expression in TE1 cells after co-transfection with sh-LINC00114 and si-DLC1; E CCK-8 detected proliferation of EC cells after co-transfection with sh-LINC00114 and si-DLC1; F colorimetry detected glucose consumption in EC cells after co-transfection with sh-LINC00114 and si-DLC1; G lactic acid determination kit II detected lactic acid production in EC cells after co-transfection with sh-LINC00114 and si-DLC1; H ATP colorimetric determination kit detected ATP level in EC cells after co-transfection with sh-LINC00114 and si-DLC1; data were shown as mean ± standard deviation. N = 3; *P < 0.05 versus the sh-NC group. # P < 0.05 versus the sh-LINC00114 + si-NC group

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