Fig. 2

Little overlap in DMPs between transgender men on masculinizing GAHT and transgender women on feminizing GAHT. A Summary of DMPs identified in transgender women and transgender men comparison at p value < 0.05 and Δβ > 0.02. B Scatter plot of change in DNA methylation during GAHT (12 months - baseline) for transgender men (x axis) and transgender women (y axis) for 64 DMPs that are significant in both GAHT groups. The largest group of DMPs (39 in total) show higher DNA methylation after 12 months of masculinizing GAHT and lower DNA methylation after 12 months of feminizing GAHT. C PCA plot of individual samples at baseline and 12 months based on the 39 anti-correlating DMPs. PC2 separates 12 months from baseline samples, with transgender women moving downwards and transgender men moving upwards. D and E Dot plot showing DNA methylation level for individual donors at baseline and 12 months at top probes that show an inverse change in DNA methylation between transgender women and transgender men groups. The probes are associated with IL21 and UBE2H genes