Fig. 5

HDAC8 silencing or inhibition sensitizes BLBC cells to chemotherapy treatment. A ROC analysis from publically available TNBC data demonstrating that patients with poor response to chemotherapy harbor high expression levels of HDAC8. Box plots: Mann–Whitney test. B, C Proliferation assay of CAF-treated pG-2 (B) and rG-2 (C) cells upon Hdac8 silencing. Representative crystal violet pictures are at the right panel of the bar charts. D Proliferation assay of pG-2 treated with low CAF doses (156.25 ng/ml cyclophosphamide, 7.8 ng/ml doxorubicin, 156.25 ng/ml 5-FU) for 48 h, w/ or w/o HDAC8 inhibition (5 μM PCI34051). E rG-2 cells treated with CAF for 48 h, w/ or w/o HDAC8 (5 μM PCI34051). Representative crystal violet pictures are at the left and right panel of proliferation kinetic graphs for pG-2 and rG-2, respectively. F Proliferation assay of HCC1806 treated with low CAF doses (156.25 ng/ml cyclophosphamide, 7.8 ng/ml doxorubicin, 156.25 ng/ml 5-FU) for 48 h, w/ or w/o HDAC8 inhibition (10 μM PCI34051). All experiments were performed in biological triplicates. ns = no difference, *p-val < 0.05, ***p-val < 0.005, ****p-val < 0.001. Statistical test: Student's t test. Error bars are standard error of the mean (SEM)