Fig. 2

Principle of QCIGISH technology and novel imprinted gene evaluation. A Illustration showing the QCIGISH principle and the respective equations used for calculating BAE, MAE and TE measurements. The QCIGISH method targets the non-coding intronic nascent RNAs to visualize the transcription loci of imprinted genes in the cell nuclei. Blue components in the image are cell nuclei stained using hematoxylin. The distinct red or brown dots represent the detected gene-expressing sites. The different allelic expressions of imprinted genes are quantified based on the transcription signals. Aberrant expressions for abnormal cells exhibit two or more dots, while normal cells contain no to a single dot. B ROC curves showing the significant differences in the AUC values determined for the BAE and MAE of HM13 as compared to the QCIGISH binary classification model during the imprinted gene selection study. *, significant differences between AUC values, p < 0.05