Fig. 1

Analysis of TET1 expression by immunohistochemistry using conventional paraffin slides and tissue microarrays (TMA) in normal prostate (NOR) and prostate carcinoma tissue (PCa). A In NOR, TET1 was expressed in CK903-positive and AMACR-negative normal basal epithelial cells; these TET1-positive cells were rare and scattered in the basal epithelium (A.1 and A.2). In PCa, TET1 was expressed in CK903-negative and AMACR-positive cancer cells, and TET1-expressing cells appeared much more frequently than in NOR (A.3); B TET1 expression was detected in both the cytoplasm (B.1) and the nucleus (B.2); C single NOR- and PCa-TMA spots were categorized according to the TET1 expression level as TET1-high (C.1, e.g., TMA-1 and TMA-2), TET1-moderate (C.2, e.g., TMA-3 and TMA-4), or TET1-negative (C.3, e.g., TMA-5 and TMA-6). The frequencies of TET1-high, moderate, and low TMA spots were calculated among NOR in comparison with PCa in regard to tumor stage (T2 to T4) and Gleason score (GS6 to GS9) (C.4)