Table 1 The stability of bisulfite converted DNA prepared by different commercially available kits
From: Guidelines for pre-analytical conditions for assessing the methylation of circulating cell-free DNA
Kit Name | Input volume, µl | Elution volume, µl | Input DNA | Optimal input DNA | Size | Recovery (%) | Efficiency (%) | Source | DNA protection | Protocol time (min) | Storage time | 96-Well format | Automation |
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
MethylEasy Xceed | 20 | 12–100 | 0.05 ng–5 µg | 0.05 ng–5 µg | – |  > 99.9 |  > 99 | Cells, tissue | Yes (Salmon sperm) | 90 |  > 3 months | Yes (beads) | – |
CpGenome™ Turbo Bisulfite Modification Kit | 10 | 25 + 25 | 0.5 ng–1 µg | 1 ng–1 µg | – | – |  > 99.9 | Cells, tissue | No | 100 |  > 2 months | No | – |
EpiTect Plus Bisulfite Conversion | 20–40 | 20 + 20 | 1 ng–2 µg | 1–2 µg | – | – |  > 99 | All kinds | Yes (carrier RNA) | 370 | 18 months | Yes | Yes |
Bisulflash™ DNA Modification Kit | 1–5 | 10–20 | 0.2 ng–1 µg | 50–200 ng |  > 100 bp, optimized for 250 bp |  > 90 |  > 99.9 | – | No | 60 | 6 months | Yes | Yes (beads) |
EpiJet Bisulfite Conversion Kit | 20 | 6–20 | 0.05 ng–2 µg | 200–500 ng | Optimized for > 50 bp | – |  > 99 | – | No | 220 | 1 year | No | Yes |
Imprint DNA Modification Kit | 10 | 8–20 | 0.1 ng–1 µg | 50–200 ng | – | – |  > 99 | – | Yes (BSAsolution) | 155 | 2 months | No | No |
EpiMark Bisulfite Conversion Kit | 10 | 20 + 20 | 50 ng–2 µg | 50 ng–2 µg | Optimized for > 150 bp | – | – | – | No | 245 | 6 months | No | No |
EZ DNA Methylation Direct Kit | 20 | 10 | 50 pg—2 µg | 200–500 ng |  > 17 bp |  > 80 |  > 99.9 | All kinds | No | 260 | 6 months | Yes | Yes (beads) |
Premium Bisulfite | 20 | 10 | 0.1 ng–2 µg | 200–500 ng | Optimized for bp > 500 |  > 80 |  > 99 | – | No | 115 | 6 months | No | Yes (column) |
Bisulflash™ DNA Bisulfite Conversion Easy Kit | 2–15 | 10–20 | 100 ng–1 µg | 100 ng |  > 100 bp, optimized for 250 bp |  > 75 |  > 99.9 | Cells, tissue | No | 80 | 6 months | Yes | No |
innuCONVERT Bisulfite Body Fluids Kit | 50 | 20–100 | 0.5 ng–10 µg | – | Optimized for > 100 bp | – | – | cirDNA in body fluids | Yes (carrier RNA) | 110 | 2 months | No | – |
Custom made Protocol | 35 | 35 | – | – |  > 17 bp | – | – | – | No | 50 | 6 months | No | Yes |