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Table 1 The stability of bisulfite converted DNA prepared by different commercially available kits

From: Guidelines for pre-analytical conditions for assessing the methylation of circulating cell-free DNA

Kit Name Input volume, µl Elution volume, µl Input DNA Optimal input DNA Size Recovery (%) Efficiency (%) Source DNA protection Protocol time (min) Storage time 96-Well format Automation
MethylEasy Xceed 20 12–100 0.05 ng–5 µg 0.05 ng–5 µg  > 99.9  > 99 Cells, tissue Yes (Salmon sperm) 90  > 3 months Yes (beads)
CpGenome™ Turbo Bisulfite Modification Kit 10 25 + 25 0.5 ng–1 µg 1 ng–1 µg  > 99.9 Cells, tissue No 100  > 2 months No
EpiTect Plus Bisulfite Conversion 20–40 20 + 20 1 ng–2 µg 1–2 µg  > 99 All kinds Yes (carrier RNA) 370 18 months Yes Yes
Bisulflash™ DNA Modification Kit 1–5 10–20 0.2 ng–1 µg 50–200 ng  > 100 bp, optimized for 250 bp  > 90  > 99.9 No 60 6 months Yes Yes (beads)
EpiJet Bisulfite Conversion Kit 20 6–20 0.05 ng–2 µg 200–500 ng Optimized for > 50 bp  > 99 No 220 1 year No Yes
Imprint DNA Modification Kit 10 8–20 0.1 ng–1 µg 50–200 ng  > 99 Yes (BSAsolution) 155 2 months No No
EpiMark Bisulfite Conversion Kit 10 20 + 20 50 ng–2 µg 50 ng–2 µg Optimized for > 150 bp No 245 6 months No No
EZ DNA Methylation Direct Kit 20 10 50 pg—2 µg 200–500 ng  > 17 bp  > 80  > 99.9 All kinds No 260 6 months Yes Yes (beads)
Premium Bisulfite 20 10 0.1 ng–2 µg 200–500 ng Optimized for bp > 500  > 80  > 99 No 115 6 months No Yes (column)
Bisulflash™ DNA Bisulfite Conversion Easy Kit 2–15 10–20 100 ng–1 µg 100 ng  > 100 bp, optimized for 250 bp  > 75  > 99.9 Cells, tissue No 80 6 months Yes No
innuCONVERT Bisulfite Body Fluids Kit 50 20–100 0.5 ng–10 µg Optimized for > 100 bp cirDNA in body fluids Yes (carrier RNA) 110 2 months No
Custom made Protocol 35 35  > 17 bp No 50 6 months No Yes