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Fig. 2 | Clinical Epigenetics

Fig. 2

From: High-throughput and affordable genome-wide methylation profiling of circulating cell-free DNA by methylated DNA sequencing (MeD-seq) of LpnPI digested fragments

Fig. 2

Principal component analysis of MeD-seq cfDNA methylation profiles using different blood tubes and input amounts. Principal components were calculated for cfDNA methylation profiles of 3 patients from which blood was collected at the same time in EDTA and CellSave tubes and either 10 ng cfDNA or the maximal input of 8 µl DNA was used. The maximal input amount was kept equal for EDTA and CellSave within 1 patient. PC1 and PC2, with the explained variances, are shown on the x-axis and y-axis, respectively. Samples from the same patient are indicated by the shapes used, where each icon represents 1 cfDNA sample (patient M4 by ▲, patient M10 by ■, and patient M19 by ●). In A, samples are coloured based on the tube the blood was collected in (EDTA in black and CellSave in red). In B, samples are coloured based on the cfDNA input amount used, 10 g in black and maximum input in red (> 30 ng)

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Clinical Epigenetics

ISSN: 1868-7083

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