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Fig. 1 | Clinical Epigenetics

Fig. 1

From: High-throughput and affordable genome-wide methylation profiling of circulating cell-free DNA by methylated DNA sequencing (MeD-seq) of LpnPI digested fragments

Fig. 1

Schematic workflow of MeD-seq on cfDNA. A The LpnPI endonuclease recognizes methylated CpG motifs and incises the DNA 16 bp upstream and downstream to generate 32 bp fragments. B After adaptor ligation, size fractionation is performed using the Pippin HT platform. C All fragments of the selected size are amplified by PCR and sequenced. Only fragments with a CpG on the central position pass the filter and are considered as methylated reads. Green: LpnPI endonuclease, black tabs: CpG-site, red circles: CpG methylation

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Clinical Epigenetics

ISSN: 1868-7083

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