Fig. 5

Integrative analysis of H3K27ac and H3K4me3 in 1-year-old stunted children. a Overview of the “gene-centric” approach in which each differential H3K27ac and H3K4me3 region was assigned to its target gene(s). Only genes predicted to be regulated by both H3K27ac and H3K4me3 were functionally annotated. b, d Venn diagrams show overlap of genes regulated by H3K27ac regions that were downregulated in stunting (red circle), and H3K4me3 regions that were downregulated (blue circle) and upregulated (grey circle) in stunting respectively. c, e Networks of biological terms as in Fig. 3b generated for overlapping genes from b, and d respectively. The color code of network edges corresponds to the color code of H3K4me3 circles in the associated Venn diagrams. f Overview of the “region-centric” approach. Significantly affected H3K27ac regions that overlap significantly affected H3K4me3 regions were assigned to target genes, which were functionally annotated. g, h Venn diagrams showing intersections of significantly downregulated H3K27ac regions (red circle) in stunting with significantly downregulated (blue circle) or upregulated (grey circle) H3K4me3 regions in stunting. i Pathway enrichment of genes associated with overlapping regions from g, h. Bars are color-coded accordingly with H3K4me3 circles in Venn diagrams