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Fig. 1 | Clinical Epigenetics

Fig. 1

From: Hi-C profiling of cancer spheroids identifies 3D-growth-specific chromatin interactions in breast cancer endocrine resistance

Fig. 1

Establishing 3D spheroids of breast normal and cancer cells. A Phase contrast images of 2D cells of MCF10A, MCF7 and MCF7TR at 20X magnification (a), of 3D spheroids at 10X magnification (b) and of 3D spheroids at 20X magnification (c), respectively. B IHC staining showing higher ER in 3D spheroids of MCF7 (MCF7_3D) and in 3D spheroids of MCF7TR (MCF7TR_3D) but absent in 3D spheroids of MCF10A (MCF10A_3D) with an imaging resolution of 100 µm or 40X magnification. C Western blotting in gel images (a) and in histograms (b) showing both ER and PR proteins were highly expressed in MCF7_2D/3D, only ER was highly expressed in MCF7TR_2D/3D, neither proteins was explicitly expressed in MCF10A_2D/3D. β-actin levels were measured as a loading control. All data in (b) corresponded to the mean ± SD of three independent experiments

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Clinical Epigenetics

ISSN: 1868-7083

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