Fig. 3

Characterization of the expression of the DMR associated genes in the scRNA-seq datasets. a Right panel: uniform manifold approximation and projection (UMAP) plot showing the germ cell subset of the scRNAseq data published in [26]. Left panel: UMAP plots showing the cells obtained from three normal controls (CTR, n = 14,098) and from the three cryptozoospermic patient samples (CZ, n = 5939). The cells are color coded according to their identity defined by the assignment published in [26]. b UMAP plot showing the integrated CTR-CZ germ cell dataset aligned along the latent time. The cells are color-coded according to their progression along the latent time. The undifferentiated spermatogonia cluster was set as starting point of the differentiation process. c Heatmap showing the normalized expression of the 55 DMR-associated genes with more than 500 counts in the CTR dataset. The cells are plotted along the latent time with the undifferentiated spermatogonia as starting point on the left side. The clustering analysis identified 5 clusters and left 12 genes unclustered. d Line plots showing the normalized expression along the latent time of the 55 DMR-associated genes with high expression grouped according to the belonging cluster in the CTR (teal) dataset