Fig. 6

Differences in the synergistic effects of cisplatin with azacytidine, decitabine, and thioguanine were observed after cotreatment of cisplatin with the three drugs on 3D spheroids of PEO1, PEO4 and OVCA433 cells by CellTiter-Glo® 3D cell viability assay. Analysis of synergism and antagonism was performed by Combenefit analysis software using the HSA model. The viability plots, HSA synergism and antagonism plots and synergy maps are shown for each treatment on the three ovarian 3D spheroids. The regions showing increasing synergism are presented from cyan to blue, while regions showing increasing antagonism are presented from yellow to red. For all, N = 3 technical replicates per sample. Data are represented as the mean ± SEM. *P < 0.05; **P < 0.01; ***P < 0.0001 (Student’s t test)