Fig. 1
From: SETD2-mediated epigenetic regulation of noncanonical Wnt5A during osteoclastogenesis
Elevated SETD2 expressions during osteoclastic differentiation. a TRAP staining was performed to detect differentiated osteoclasts at days 4 and 6. b Relative gene expression of Nfatc1, Trap, Cathepsin K, and Mmp9 were determined in differentiated cells using qRT-PCR keeping β-Actin as an internal control. c The relative gene expression of Setd2 was determined in differentiated cells. d Protein levels of SETD2 were detected by western blot and the protein expression level was analyzed by ImageJ software. The protein expression level was normalized by using GAPDH expression level and graphically represented. e Imunofluorescence analysis of SETD2 expression in osteoclast differentiated samples