Fig. 5

Correlation of NEFM transcriptional expression/DNA methylation with immune infiltration levels in BRCA. a NEFM transcriptional expression weakly positively correlated to infiltration levels of CD8 + T cells, CD4 + T cells and macrophages in BRCA in TIMER database by correlation analysis. NEFM expression showed very weak positive association with infiltration levels of neutrophils and dendritic cells in TCGA BRCA in TIMER database (n = 703). b DNA methylation of NEFM had significant negative association with infiltration levels of B cells, CD8 + T cells, CD4 + T cells, macrophages, neutrophils, and dendritic cells in TIMER database by correlation analysis (n = 703). c NEFM expression had weak positive association with infiltration levels of activated B cells, macrophages and moderate positive association with infiltration levels of neutrophils, whereas negative association with infiltration levels of activated CD8 + T cells, activated CD4 + T cells and activated dendritic cells in BRCA in TISIDB database (n = 1100). d DNA methylation of NEFM significantly negatively correlated with infiltration levels of activated B cells, activated CD8 + T cells, activated CD4 + T cells, macrophages, neutrophils, and activated dendritic cells in TISIDB database (n = 785). e NEFM expression weakly negatively correlated with infiltration levels of M2 macrophage; DNA methylation of NEFM negatively correlated with infiltration levels of M1 macrophage whereas positively correlated with infiltration levels of M2 macrophage in TIMER database (n = 703). f NEFM transcriptional expression positively correlated with infiltration levels of CD8 + T cells, macrophages, neutrophils, dendritic cells and negatively correlated with infiltration level of B cells in TIMER gene modules (n = 1100). e After adjusted by tumor purity, NEFM expression weekly negatively correlated with infiltration level of B cell and positively correlated with infiltration levels of macrophages and CD8 + T cells in TIMER gene modules (n = 1100)