Fig. 4

Performance of the two markers in the Wuhan clinical sample set using the TAM-MSP method. a The two markers, cg0504 and SCG3, assessed as a panel or assessed individually, achieved 100% accuracy in distinguishing PCNSL from the 8 other CNS tumors. b The histogram of cumulative methylation for the two-marker panel in each sample displays the contribution of the two markers in detecting PCNSL among the CNS tumors in the sample set. c Analysis of receiver operator characteristics (ROC, inset) shows that the two-marker panel performed with a high degree of accuracy with AUC of 1.00 (CI 0.95–1.00). **Mann–Whitney P < 0.001; CMI cumulative methylation index, N number of samples, P Mann–Whitney statistics, AUC area under the curve