Table 4 Sequences of the primers used and annealing temperature for each gene
From: Epigenetic landscape of testis specific histone H2B variant and its influence on sperm function
Gene | Forward primer | Reverse primer | Annealing temp. (°C) |
|---|---|---|---|
CREM | CCACATTACTGAATATGGGTGCT | TTTCTAAGTGCAGAAACATGCCT | 58 |
CDYL | GGACTTCAAAGTTGGGGGCA | GAGGGTCTGTCAGCTTGTGA | 60 |
PRKAG2 | TGAGCCTTCAGTGAGTGGTA | CCATCCTAACCTTCAGGAAGCA | 60 |
CATSPERB | GCACCGTATGGTGTGGACTA | CCCTCCAGACAAAGCACCAT | 60 |
TSGA10 | GGTGTCTGCTAATTGCCAGGT | GATAGGAGGTTTGGGCCACG | 60 |
TSSK1B | CCAACGGGATCTTGCTGAAC | AGCATATCCATACGCAGAACCAT | 62 |