Fig. 2

Characterization of BC DNA methylation signatures associated with BC and binary model development in Cohort 1. a Unsupervised hierarchical clustering of 22 DNA methylation markers; differential methylation profiles were represented reversely by ∆Ct of each marker. b Performance features of top individual markers for BC detection in single marker analysis; AUC, sensitivity and specificity were expressed as mean with 95% CI in 2000 bootstrap samplings; groups of clustering were sorted from unsupervised hierarchical clustering. c Top marker correlation matrix and unsupervised hierarchical clustering. d ROC curves of dual-marker detection model. e Distributions of dual-marker model BC risk probability in non-BC and BC groups; unpaired t test was used to analyze statistical significance; ****p < 0.0001