Fig. 1

HDAC2 is up-regulated in ESCC tissues and cells, and connected with inferior prognosis. a RT-qPCR to detect HDAC2 mRNA expression in ESCC cancer and normal tissues; b Western blot to detect HDAC2 protein expression in ESCC cancer and normal tissues; c RT-qPCR to detect HDAC2 mRNA expression in ESCC cell lines and HEEC; d Log-rank test to analyze survival prognosis of ESCC patients with high and low HDAC2 expression levels; for all quantitative results, the data were expressed as mean ± standard deviation of three independent experiments. Discrepancies between two groups were assessed by Student’s t test while those among multiple groups by one-way ANOVA; * P < 0.05 compared with HEEC cells