Fig. 2

Bisulfite–polymerase chain reaction (PCR) sequencing of the SERPINA1 promoter of DNAs from blood samples belonging to acute coronary syndrome (ACS) patients a with chronic obstructive pulmonary disease (COPD+) and b without COPD (COPD−). In both panels: stratification of bisulfite–PCR sequences according to ACS subgroup and SERPINA1 hyper- and hypomethylation status. Filled-in and clear circles represent methylated and unmethylated CpGs, respectively. The CpGs within the SERPINA1 promoter are numbered across the top of the grid. Each row represents one PCR product/analyzed sequence. Samples showing more than or equal to 5 out of 8 (≥ 50%) methylated CpGs were considered hypermethylated; c frequencies of SERPINA1 promoter hypermethylation in COPD+ and COPD− samples. *p < 0.05