Fig. 4

Effect of CBP downregulation by siRNA on the expression of ERα in MCF7 and T47D cells. a, b Western blot analysis of CBP and ERα proteins in a MCF7 and b T47D cells transfected with negative control (NC) or CBP siRNA for 24–96 h. The graphs show the relative amounts of ERα protein normalized to β-actin. Fold change of ERα expression was done over cells transfected with NC siRNA for each time point. Full blots images are available in Additional file 1: Fig. S10. Average present as mean ± SEM (n = 3). *p < 0.05 versus untreated control, unpaired t test